Biochemistry and Genetics: Pretest Self-Assessment and by Wilson G.N., Schroeder B., Woo J.

By Wilson G.N., Schroeder B., Woo J.

Those "500 questions, solutions, and clarification" books are designed to simulate the us scientific Licensing exam (USMLE) Step 1. each one is authored by way of knowledgeable within the box and has been reviewed via scholars who've lately handed the examination. As Step 1 is turning into a growing number of medical in nature, while appropriate, scientific vignette-style questions requiring a two-step reasoning method also are incorporated. the entire Pretest titles comprise a "high-yield" truth part to augment key suggestions.

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Sample text

Scriver, pp 3–45. Sack, pp 3–29. ) A special DNA polymerase called telomerase is responsible for replication of the telomeric DNA. Telomerase contains an RNA molecule that guides the synthesis of complementary DNA. Telomerase is therefore an RNA-dependent DNA polymerase in a category with reverse transcriptase. Telomerase does not require an RNA primer, initiating synthesis of the leading strands at 3′ ends within the telomeric DNA. Synthesis of the lagging strands uses primase, DNA polymerase III, and DNA polymerase I, as with the replication of other chromosomal regions.

The DNA-RNA helix formed binds DNA polymerase III, which synthesizes a DNA fragment (the Okazaki fragment) in a 5′ to 3′ direction. When the RNA primer of the previous Okazaki fragment is met, DNA polymerase I replaces III and digests the RNA primer, replacing it with appropriate DNA bases. When the RNA primer is completely removed, DNA ligase synthesizes the last phosphodiester bond, thereby sealing the space. What is left is a new lagging strand extended by the new Okazaki fragment with the 10-base RNA primer at its 5′ end.

Template-directed RNA synthesis occurs in which of the following? a. b. c. d. e. Point mutation Triplet repeat expansion Initiation of the polymerase chain reaction Expression of oncogenes Repair of thymine dimers 52. Which of the following most correctly describes mammalian messenger RNAs? a. b. c. d. e. They are usually transcribed from both DNA strands They are normally double-stranded Their content of uridine equals their content of adenine They have an overall negative charge at neutral pH Their ratio of ribose to purine bases equals 1 53.

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