Advances in Lipid Methodology. Volume 3 by W. W. Christie

By W. W. Christie

This can be the 3rd quantity of an occasional sequence of overview volumes facing features of lipid technique. As with the 1st volumes, subject matters were chosen which were constructing swiftly in recent times and feature a few significance to lipid research. The authors are all top foreign experts.

Topics coated comprise: research of positional isomers of glycerolipids through non-enzymatic equipment, separation of phospholipid periods by means of high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid part behaviour and lipid diffusion, between others

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Kuksis. 1990. Unpublished results]. Instrument and chiral column was as given in Fig. 7. ); flow rate, 1 mL/min at 25°C. Peak identification: 1, sn-1-acetyl-2-palmitoylglycerol; 2, sn-1palmitoyl-2-acetylglycerol; 3, sn-2-palmitoyl-3-acetylglycerol; 4, sn-2-acetyl-3-palmitoylglycerol. the 3,5-DNPU derivatives. in two solvent systems. 9A). 9B), yet the reverse isomers of the sn-2,3-enantiomers were resolved. In both enantiomer classes the isomers with the acetyl chain in the primary position were eluted first.

66. E. , Lipids, 5, 48-53 (1990). 67. , Buchnea,D. ,J. Biol. , 250, 3414-3418 (1975). 68. , In Handbook of Lipid Research, Vol. 1, Fatty Acids and Gklycerides, pp. 123-196 (1978) (edited by A. Kuksis, Plenum Press, Inc, New York). 69. J. , Can. J. , 57, 117-124 (1979). 70. , Marai,L. , Anal. , 50, 557-561 (1978). 71. , Yang,L-Y. , Biochem. , 65, 811-821 ( 1987). 72. N. , Lipids, 25, 33-39 (1990). 73. , Lipids, 19, 56-59 (1984). 74. , Lipids, 20, 778-782 (1985). 75. , Lipids, 23, 140-143 (1988).

2 ml per ml chloroform/methanol) is added, the funnel shaken vigorously, and the two liquid phases allowed to separate overnight. The lower chloroform phase is collected and evaporated on a rotary evaporator at 37°C. The concentrated and dried lipid sample is then taken up in chloroform and an appropriate quantity used for 31 P NMR analysis. The extraction of body fluids, such as human urine [11 ], amniotic fluid, cyst fluid, plasma, synovial fluid, or cerebrospinal fluid, may require further modification.

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