By Felix R. Althaus, Hanna E. Kleczkowska (auth.), Rafael Alvarez-Gonzalez (eds.)
This specified factor of Molecular and mobile Biochemistry comprises twenty-two chosen learn papers and experiences from a complete of 1 hundred and ten shows given on the twelfth overseas Symposium on ADP-ribosylation Reactions: From Bacterial Pathogenesis to melanoma, held in Cancun, Mexico, could 10-14, 1997. The Symposium was once hosted via the Sociedad Mexicana de Bioquimica and used to be backed by way of the college of North Texas future health technological know-how heart, fortress worthy, TX, united states.
This quantity presents a state of the art resource of data for easy scientists and clinicians who're drawn to the molecular, biochemical, and mobile features of protein-(ADP-ribose) move reactions in human well-being and disease.
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Extra info for ADP-Ribosylation Reactions: From Bacterial Pathogenesis to Cancer
G. oncogenes or drug resistance genes, which play an important role in carcinogenesis. We have previously shown that the chemical ADP-ribosylation inhibitor 3-aminobenzamide potentiates Simian virus 40 (SV 40) DNA amplification induced by the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in the Present address: J-H Kiipper, Universitat Tiibingen, Institut fiir Pathologie, Liebermeisterstr. 8, D-72076 Tiibingen, Germany Address for offprints: A. Biirkle, DKFZ, Abteilung FOlOO, 1m Neuenheimer Feld 242, D-69120 Heidelberg, Germany 32 SV40-transformed hamster cell line C060 .
K. Simons (EMBL, Heidelberg, Germany). Ilimaquinone was a gift from Dr. V. Malhotra (University of California, San Diego, CA, USA). The P5D4 mAb against the G protein of VSV was obtained from Sigma (St. Louis, MO, USA). Rabbit anti-a-mannosidase II (Man II) antibody was kindly provided by Dr. K. Moremen (University of Georgia, Athens, GA, USA). All other chemicals were obtained from commercial sources at the highest available purity. Cell permeabilization ° RBL and CHO cells were permeabilized by 1 U/ml of streptolysin (SLO, Biomerieux, Marcy l'Etoile, France) as previously descibed .
Nature 376: 37-43, 1995 13. Negri C, Donzelli M, Bernardi R, Rossi L, Biirkle A, Scovassi AI: Multiparametric staining to identify apoptotic human cells. Exp Cell Res 234: 174-177, 1997 14. Ding R, Smulson M: Depletion of nuclear poly(ADP-ribose) polymerase by antisense RNA expression: influences on genetic stability, chromatin organization, and carcinogen cytotoxicity. Cancer Res 54: 4627-4634, 1994 15. Schreiber V, Hunting D, Trucco C, Gowans B, Grunwald D, de Murcia G, Menissier-deMurcia J: A dominant-negative mutant of human poly(ADP-ribose) polymerase affects cell recovery, apoptosis, and sister chromatid exchange following DNA damage.